Off-target effects in CRISPR/Cas9 gene editing

Off-target effects in CRISPR/Cas9 gene editing

Blog Article

Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence.With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient, convenient and programmable, leading to promising translational studies and clinical trials for both genetic and non-genetic diseases.A major concern in the applications of abc material the CRISPR/Cas9 system is about its off-target effects, namely the deposition of unexpected, unwanted, or even adverse alterations to the genome.To date, many methods have been developed to nominate or detect the off-target sites click here of CRISPR/Cas9, which laid the basis for the successful upgrades of CRISPR/Cas9 derivatives with enhanced precision.

In this review, we summarize these technological advancements and discuss about the current challenges in the management of off-target effects for future gene therapy.